Part:BBa_K2175005:Design

K592024 BFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 2
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 120
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Antisense chain of coding region of BFP

Source

gaattcgcggccgcttctagagctcgagcaccaccaccaccactgcatggttaattcctcctagccgctggacgacagccaagtaagatattgcttagctaaatccgcacctgctgccgaagcacctggcactatgcatcgccggcccaatgcactatacagatacgaccttagacgttaccggacgttgcggcaagcgtgacttaaggtaagtcaggcaggggatgtgggccgcgacgacctcaaggtgatctcgcggatatccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagtagcggccgctgcag